Technical Data
0004-01J
4-1BB LIGAND, (TNFSF9, 4-1BBL, 4-1BB-L, Tumor necrosis factor ligand superfamily member 9), Recombinant Human
20ug
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Technical Data |
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0004-01J |
4-1BB LIGAND, (TNFSF9, 4-1BBL, 4-1BB-L, Tumor necrosis factor ligand superfamily member 9), Recombinant Human |
20ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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4-1BB Ligand (4-1BBL/TNFSF9) is preferentially expressed by B cells, and is a ligand for CD137 (4-1BB ligand receptor/TNFRSF9). Interaction between 4-1BBL and its receptor acts as a co-stimulatory signal for T cell activation and expansion, and is involved in the generation of cytotoxic T cells and in the process of antigen presentation. Expression of 4-1BBL by a variety of carcinoma cells, suggests a role for 4-1BBL in tumor cell-T cell interactions. Source: Purified recombinant 4-1BBL expressed in E.coli Activity: Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The ED50 for this effect is 5-10 ng/ml. Please note: Results may vary with PBMC donors. Applications: Suitable for use in Functional Assays. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Endotoxin: 1EU/ug. Storage and Stability: Lyophilized powder may be stored at-20°C. Stable for 12 months at -20°C. Reconstitute with 200ul dH2O. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 19.5kD |
Source: E. coli. Purity: 98% by SDS PAGE and HPLC analysis. Concentration: ~0.1mg/ml Form: Supplied as a lyophilized powder in PBS, pH 7.2, 1% BSA.. Reconstitute with 200ul dH2O. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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