Technical Data
0004-01J
4-1BB LIGAND, (TNFSF9, 4-1BBL, 4-1BB-L, Tumor necrosis factor ligand superfamily member 9), Recombinant Human
20ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
4-1BB Ligand (4-1BBL/TNFSF9) is preferentially expressed by B cells, and is a ligand for CD137 (4-1BB ligand receptor/TNFRSF9). Interaction between 4-1BBL and its receptor acts as a co-stimulatory signal for T cell activation and expansion, and is involved in the generation of cytotoxic T cells and in the process of antigen presentation. Expression of 4-1BBL by a variety of carcinoma cells, suggests a role for 4-1BBL in tumor cell-T cell interactions.

Source:
Purified recombinant 4-1BBL expressed in E.coli

Activity:
Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The ED50 for this effect is 5-10 ng/ml. Please note: Results may vary with PBMC donors.

Applications:
Suitable for use in Functional Assays. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Endotoxin:
1EU/ug.

Storage and Stability:
Lyophilized powder may be stored at-20C. Stable for 12 months at -20C. Reconstitute with 200ul dH2O. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
19.5kD
Source: E. coli.
Purity: 98% by SDS PAGE and HPLC analysis.
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder in PBS, pH 7.2, 1% BSA.. Reconstitute with 200ul dH2O.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.