		Technical Data
0004-01Q	4-1BB Ligand (TNFSF9, CD137, CDw137, ILA, MGC2172, T-cell, 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9 precursor) (Biotin)

Description:
4-1BB, also known as CD137, is a type I transmembrane protein belonging to the TNF
receptor superfamily (TNFRSF9). It is expressed on T cells, NK cells, monocytes,
neutrophils, dendritic cells, and eosinophils. The ligand for 4-1BB, 4-1BBL, is a TNF
ligand superfamily member (TNFSF9) expressed on the surfaces of activated antigen
presenting cells and T and B lymphoma cell lines. 4-1BB transduces a T cell
costimulatory signal to promote survival, proliferation, cytokine production, and effector
functions. On dendritic cells, 4-1BB ligation enhances cytokine production and
upregulates expression of B7-1 and B7-2 costimulatory molecules.

Applications:
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 3-10ug/ml/10
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Goat

Concentration:

~50ug/ml

Immunogen:

Recombinant human 4-1BB Ligand aa71-254.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with BSA. Reconstitute with 40-50% glycerol, PBS.

Specificity:

Recognizes human 4-1BB Ligand/TNFSF9.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.