Technical Data
0004-01R
4-1BB Ligand (TNFSF9, CD137, CDw137, ILA, MGC2172, T-cell, 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9 precursor)
Description:
4-1BB, also known as CD137, is a type I transmembrane protein belonging to the TNF
receptor superfamily (TNFRSF9). It is expressed on T cells, NK cells, monocytes,
neutrophils, dendritic cells, and eosinophils. The ligand for 4-1BB, 4-1BBL, is a TNF
ligand superfamily member (TNFSF9) expressed on the surfaces of activated antigen
presenting cells and T and B lymphoma cell lines. 4-1BB transduces a T cell
costimulatory signal to promote survival, proliferation, cytokine production, and effector
functions. On dendritic cells, 4-1BB ligation enhances cytokine production and
upregulates expression of B7-1 and B7-2 costimulatory molecules.

Applications:
Suitable for use in RIA, Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 3-10ug/ml/10
ELISA: 0.5-1.0ug/ml
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.2mg/ml
Immunogen:
Recombinant human 4-1BB Ligand aa71-254 (E. coli).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in 0.2 um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.5ml of PBS is used, the antibody concentration will be 0.2mg/ml.
Specificity:
Recognizes 4-1BB Ligand/TNFSF9.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.