Technical Data
0004-01T
4-1 BB Ligand, Recombinant, Human, aa71-254, His-Tag (4-1BBL, CD137L, TNFSF9, Tumor necrosis factor ligand superfamily member 9)
50ug
100ug
250ug
Growth Factors, Cytokines Storage: -70CShipping: Blue Ice
Human 4-1BB Ligand (4-1BBL, CD137L) is a 32kD type II transmembrane protein that belongs to TNF superfamily (TNFSF) of molecules.1-4 The human 4-1BBL cDNA encodes a 254 amino acid (aa) protein that contains a 25aa N-terminal cytoplasmic domain, a 23aa transmembrane segment, and a 206aa C-terminal extracellular region.5 The extracellular domain (ECD) of 4-1BBL has a jelly-roll, -sandwich tertiary structure that is similar to other TNFSF members. There is only one cysteine in the human ECD, and no potential N-linked glycosylation sites. The potential exists, however, for O-linked glycosylation. The human 4-1BBL ECD shares 32% and 35% aa identity with mouse and rat ECD, respectively. In the cytoplasmic domain, human 4-1BBL is 55aa shorter than the equivalent region in rodents. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated Tcells, neurons and astrocytes.2, 3, 6 A 26kD soluble form of 4-1BBL is known to occur in human. Although it is presumably generated by MMP activity, its amino acid size is currently unreported.4 The soluble form is bioactive. Human 4-1BBL signals through both CD137/4-1BB and itself. Its cytoplasmic tail participates in reverse signaling that induces apoptosis in T cells and cytokine secretion (IL-6; TNF- ) by monocytes.7, 8 4-1BBL binding to CD137/4-1BB produces a number of effects. Its seems to play a key role in the T cell recall response. It maintains T cell numbers at the end of a primary response, and induces CD4+ and CD8+ T cells to proliferate and secrete cytokines such as IL-2 and IFN- in CD4+ cells, and IFN- in CD8+ cells.9, 10

Source:
Recombinant human 4-1BB ligand, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Molecular Weight: 23.8kD (222aa)

Concentration: ~1mg/ml (determined by Bradford assay,15% SDS-PAGE (3ug)

Sequence:
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRE GPELSPDDPA GLLDLRQGMF AQLVAQNVLL IDGPLSWYSD PGLAGVSLTG
GLSYKEDTKE LVVAKAGVYY VFFQLELRRV VAGEGSGSVS LALHLQPLRS AAGAAALALT VDLPPASSEA RNSAFGFQGR LLHLSAGQRL
GVHLHTEARA RHAWQLTQGA TVLGLFRVTP EIPAGLPSPR SE

Storage and Stability:
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Source: Recombinant, human from E. coli
Purity: 95%, as determined by SDS-PAGE and visualized by silver stain.
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8, 100mM sodium chloride, 20% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
General References:
1. Kwon, B. et al., 2003, Exp. Mol. Med. 35:8.
2. Salih, H.R. et al., 2002, Int. J. Clin. Pharmacol. Ther. 40:348.
3. Vinay, D.S. and B.S. Kwon, 1998, Semin. Immunol. 1:481.
4. Salih, H.R. et al., 2001, J. Immunol. 167:4059.
5. Alderson, M.R. et al., 1994, Eur. J. Immunol. 24:2219.
6. Reali, C. et al., 2003, J. Neurosci. Res. 74:67.
7. Michel, J. et al., 1999, Immunology 98:42.
8. Langstein, J. et al., 1998, J. Immunol. 160:2488.
9. Wen, T. et al., 2002, J. Immunol. 168:4897.
10. Dawicki, W. and T.H. Watts, 2004, Eur. J. Immunol. 34:743.
11. Lee SW., et al. (2008). Nat Immunol. 9(8):917-26
12. Sharma RK., et al. (2009). Cancer Res. 69(10):4319-26

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.