4-1 BB Ligand, Recombinant, Human, aa71-254, His-Tag (4-1BBL, CD137L, TNFSF9, Tumor necrosis factor ligand superfamily member 9)
|Growth Factors, Cytokines||Storage: -70°CShipping: Blue Ice|
Human 4-1BB Ligand (4-1BBL, CD137L) is a 32kD type II transmembrane protein that belongs to TNF superfamily (TNFSF) of molecules.1-4 The human 4-1BBL cDNA encodes a 254 amino acid (aa) protein that contains a 25aa N-terminal cytoplasmic domain, a 23aa transmembrane segment, and a 206aa C-terminal extracellular region.5 The extracellular domain (ECD) of 4-1BBL has a jelly-roll, -sandwich tertiary structure that is similar to other TNFSF members. There is only one cysteine in the human ECD, and no potential N-linked glycosylation sites. The potential exists, however, for O-linked glycosylation. The human 4-1BBL ECD shares 32% and 35% aa identity with mouse and rat ECD, respectively. In the cytoplasmic domain, human 4-1BBL is 55aa shorter than the equivalent region in rodents. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated Tcells, neurons and astrocytes.2, 3, 6 A 26kD soluble form of 4-1BBL is known to occur in human. Although it is presumably generated by MMP activity, its amino acid size is currently unreported.4 The soluble form is bioactive. Human 4-1BBL signals through both CD137/4-1BB and itself. Its cytoplasmic tail participates in reverse signaling that induces apoptosis in T cells and cytokine secretion (IL-6; TNF- ) by monocytes.7, 8 4-1BBL binding to CD137/4-1BB produces a number of effects. Its seems to play a key role in the T cell recall response. It maintains T cell numbers at the end of a primary response, and induces CD4+ and CD8+ T cells to proliferate and secrete cytokines such as IL-2 and IFN- in CD4+ cells, and IFN- in CD8+ cells.9, 10
Recombinant human 4-1BB ligand, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Molecular Weight: 23.8kD (222aa)
Concentration: ~1mg/ml (determined by Bradford assay,15% SDS-PAGE (3ug)
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRE GPELSPDDPA GLLDLRQGMF AQLVAQNVLL IDGPLSWYSD PGLAGVSLTG
GLSYKEDTKE LVVAKAGVYY VFFQLELRRV VAGEGSGSVS LALHLQPLRS AAGAAALALT VDLPPASSEA RNSAFGFQGR LLHLSAGQRL
GVHLHTEARA RHAWQLTQGA TVLGLFRVTP EIPAGLPSPR SE
Storage and Stability:
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Source: Recombinant, human from E. coli
Purity: 95%, as determined by SDS-PAGE and visualized by silver stain.
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8, 100mM sodium chloride, 20% glycerol.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Kwon, B. et al., 2003, Exp. Mol. Med. 35:8.
2. Salih, H.R. et al., 2002, Int. J. Clin. Pharmacol. Ther. 40:348.
3. Vinay, D.S. and B.S. Kwon, 1998, Semin. Immunol. 1:481.
4. Salih, H.R. et al., 2001, J. Immunol. 167:4059.
5. Alderson, M.R. et al., 1994, Eur. J. Immunol. 24:2219.
6. Reali, C. et al., 2003, J. Neurosci. Res. 74:67.
7. Michel, J. et al., 1999, Immunology 98:42.
8. Langstein, J. et al., 1998, J. Immunol. 160:2488.
9. Wen, T. et al., 2002, J. Immunol. 168:4897.
10. Dawicki, W. and T.H. Watts, 2004, Eur. J. Immunol. 34:743.
11. Lee SW., et al. (2008). Nat Immunol. 9(8):917-26
12. Sharma RK., et al. (2009). Cancer Res. 69(10):4319-26