Technical Data
4-1BB (TNFRSF9, tumor necrosis factor receptor superfamily, member 9, 4-1BB, 4-1BB ligand receptor, CD137, CDw137, ILA, MGC2172, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9) (PE)
4-1BB, also known as CD137 and ILA (induced by lymphocyte activation), is a TNF receptor superfamily member and has been designated TNFRSF9. Mouse 4-1BB cDNA encodes a 256aa residue type I transmembrane protein.1-3 A soluble 4-1BB is released from the surfaces of cells expressing the transmembrane protein.4 Mouse 4-1BB shares approximately 60% aa sequence identity with its human counterpart. 4-1BB is expressed on activated CD4+ and CD8+ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, dendritic cells (DCs), and eosinophils.5 The ligand for 4-1BB (4-1BBL), also named TNFSF9, belongs to the TNF ligand superfamily. In vivo, the co-stimulatory activity of 4-1BB has been shown to be important in graft-vs-host disease and antiviral CTL responses. On dendritic cells, 4-1BB is a DC-activating molecule that enhances cytokine production and up-regulates expression of B7-1 and B7-2 co-stimulatory molecules, resulting in an improved ability to stimulate T cell responses.1-5.

Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Myeloma cells with B cells from Balb/c mice.

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
MabIgG2a11D95Affinity Purified
100Tests4C Do Not FreezeDry IceMouseRat
Not determined
Recombinant corresponding to mouse 4-1BB/TNFRSF9 extracellular domain expressed in NSO cells (P20334).
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, 0.1% sodium azide, 0.5% BSA. Labeled with R-Phycoerythrin (PE).
Recognizes mouse 4-1BB/TNFRSF9.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Goodwin, R.G. et al. (1993) Eur. J. Immunol. 23:2631. 2. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219. 3. Kwon, B.S. & S.M. Weissman (1989) Proc. Nat. Acad. Sci. USA 86:1963. 4. Wilcox, R.A. et al. (2002) J. Immunol. 168:4262. 5. Kwon, B. et al. (2002) Trends Immunol. 23:378.