		Technical Data
0004-01X	4-1BB (TNFRSF9, tumor necrosis factor receptor superfamily, member 9, 4-1BB, 4-1BB ligand receptor, CD137, CDw137, ILA, MGC2172, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9) (PE)

Description:
4-1BB, also known as CD137 and ILA (induced by lymphocyte activation), is a TNF receptor superfamily member and has been designated TNFRSF9. Mouse 4-1BB cDNA encodes a 256aa residue type I transmembrane protein.1-3 A soluble 4-1BB is released from the surfaces of cells expressing the transmembrane protein.4 Mouse 4-1BB shares approximately 60% aa sequence identity with its human counterpart. 4-1BB is expressed on activated CD4+ and CD8+ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, dendritic cells (DCs), and eosinophils.5 The ligand for 4-1BB (4-1BBL), also named TNFSF9, belongs to the TNF ligand superfamily. In vivo, the co-stimulatory activity of 4-1BB has been shown to be important in graft-vs-host disease and antiviral CTL responses. On dendritic cells, 4-1BB is a DC-activating molecule that enhances cytokine production and up-regulates expression of B7-1 and B7-2 co-stimulatory molecules, resulting in an improved ability to stimulate T cell responses.1-5.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Myeloma cells with B cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Mab	IgG2a	11D95	Affinity Purified

Size		Storage	Shipping	Source	Host
100 Tests		4°C Do Not Freeze	Dry Ice	Mouse	Rat

Concentration:

Not determined

Immunogen:

Recombinant corresponding to mouse 4-1BB/TNFRSF9 extracellular domain expressed in NSO cells (P20334).

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 0.1% sodium azide, 0.5% BSA. Labeled with R-Phycoerythrin (PE).

Specificity:

Recognizes mouse 4-1BB/TNFRSF9.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Goodwin, R.G. et al. (1993) Eur. J. Immunol. 23:2631. 2. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219. 3. Kwon, B.S. & S.M. Weissman (1989) Proc. Nat. Acad. Sci. USA 86:1963. 4. Wilcox, R.A. et al. (2002) J. Immunol. 168:4262. 5. Kwon, B. et al. (2002) Trends Immunol. 23:378.