Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: Neat Use 10ul to label 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
Recommended Negative Controls:
I1904-79D IgG1 Murine Negative Control (PE)
Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
|100Tests||4°C Do Not Freeze||Blue Ice||Human||Mouse|
| Not determined.|
|Purified by Protein G affinity chromatography.|
|Supplied as a llyophilized powder from PBS, pH 7.2, 0.1% sodium azide, 1% BSA. Labeled with R. Phycoerythrin (RPE). Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.|
|Recognizes the human CDw137 cell surface antigen, also known as 4-1BB.CDw137 is not expressed by resting peripheral blood T cells, but is expressed following activation by PHA or anti-CD3.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Garni-Wagner, B.A. et al. (1996). 4-1BB is expressed on the CD45RAhi ROhi transitional T cell in humans. Cell Immunol. 169: 91-98.|