Technical Data
0004-12
4-1 BB (CDw137) (PE)
Description:
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat Use 10ul to label 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.

Recommended Negative Controls:
I1904-79D IgG1 Murine Negative Control (PE)

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H491Affinity Purified
SizeStorageShippingSourceHost
100Tests4C Do Not FreezeBlue IceHumanMouse
Concentration:
Not determined.
Immunogen:
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a llyophilized powder from PBS, pH 7.2, 0.1% sodium azide, 1% BSA. Conjugated to R. Phycoerythrin (RPE). Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.
Specificity:
Recognizes the human CDw137 cell surface antigen, also known as 4-1BB.CDw137 is not expressed by resting peripheral blood T cells, but is expressed following activation by PHA or anti-CD3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Garni-Wagner, B.A. et al. (1996). 4-1BB is expressed on the CD45RAhi ROhi transitional T cell in humans. Cell Immunol. 169: 91-98.