Technical Data
0004-18E
4E Binding Protein 1, Blocking Peptide (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Peptide is used to block 4E-BP1 Rabbit mAb reactivity in immunohistochemistry protocols.

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the eIF4E translation initiation factor. Hyperphosphorylation of 4E-BP1 disrupts this interaction and cap-dependent translation is activated (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR on Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks 4E-BP1 Rabbit mAb signal in immunohistochemistry.

Directions for Use: For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100ul total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity: Highly Purified
Form: Supplied as a liquid in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
(1) Pause, A. et al. (1994) Nature 371, 762767. (2) Brunn, G.J. et al. (1997) Science 277, 99101. (3) Gingras, A.C. et al. (1998) Genes Dev. 12, 502513. (4) Fadden, P. et al. (1997) J. Biol. Chem. 272, 1024010247. (5) Gingras, A.C. et al. (1999) Genes Dev. 13, 14221437.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.