Technical Data
0004-19M
4E-BP1, phosphorylated (Thr70) (4EBP1, 4E-BP1, Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)
Description:
4E-BP1 is a eukaryotic translation initiation factor (eIF4E- binding protein), also known as PHAS-1. The binding of 4E-BP to eIF4E is regulated by phosphorylation and is reversible. Non-phosphorylated 4E-BP1 binds strongly to eIF4E; however, phosphorylated 4E-BP1 will not bind to eIF4E. Akt, TOR, MAP, S6, and Cdc2 kinases are able to inactivate 4E-BP1 by phosphorylation. 4E-BP1 undergoes phosphorylation which releases it from eIF4E, ultimately forming initiation complexes that aid in the upregulation of protein synthesis.

Applications:
Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1000
Optimal dilutions to be determined by the researcher.

Positive Control:
293T cell lysate.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10F619Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Phospho-specific peptide corresponding to residues surrounding Thr70 of human 4E-BP1.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Specificity:
Recognizes human phospho-4E-BP1 (Thr70).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.