Technical Data
0004-20A
4E Binding Protein 1, phosphorylated (Thr70) (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)
Description:
4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyperphosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues; phosphorylation by FRAP/mTOR on Thr37 and Thr46 of human 4E-BP1 may prime it for subsequent phosphorylation at sites including Ser65 and Thr70.

Applications:
Suitable for use in Western Blot, and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blotting: 1:1000 (Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking.)
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Molecular Weight: 15-20kD

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
Not determined
Immunogen:
Synthetic phosphopeptide derived from the sequence of mouse 4E-BP1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA 50% glycerol. No preservative added.
Specificity:
Detects endogenous levels of 4E-BP1 only when phosphorylated at Thr70. Does not detect 4E-BP1 phosphorylated at other sites. Species Cross-Reactivity: human, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Pause, A. et al. (1994) Nature 371, 762767. (2) Brunn, G.J. et al. (1997) Science 277, 99101. (3) Gingras, A.C. et al. (1998) Genes Dev. 12, 502513. (4) Fadden, P. et al. (1997) J. Biol. Chem. 272, 1024010247. (5) Gingras, A.C. et al. (1999) Genes Dev. 13, 14221437.