Technical Data
0014-02D
14-3-3, beta, Recombinant, Human (Tyrosine 3-monooxygenase/tryptopan 5-monooxygenase activation protein, beta polypeptide)
100ug
Molecular Biology Storage: -20CShipping: Dry Ice
The 14-3-3 family plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 beta, a subtype of the 14-3-3 proteins, was found in B Cells, brain and liver etc. This 14-3-3 beta has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery.

Source:
Synthetic corresponding to aa1-246 of human 14-3-3 beta expressed in E .coli.

AA Sequences:
MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY KNVVGARRSS
WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL ELLDKYLIPN ATQPESKVFY
LKMKGDYFRY LSEVASGDNK QTTVSNSQQA YQEAFEISKK EMQPTHPIRL GLALNFSVFY
YEILNSPEKA CSLAKTAFDE AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD
AGEGEN

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
28kD
Source: Synthetic corresponding to aa1-246 of human 14-3-3 beta expressed in E .coli.
Purity: 90% by SDS-PAGE
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20mM Tris pH 8.0, 1mM EDTA, 50mM NaCl.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Rodriguez LG., et al. (2005). J Cell Physiol. Jan; 202(1):285-94. 2. Mils V., et al.(2000). Oncogene. Mar 2; 19(10):1257-65.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.