Technical Data
0014-02D
14-3-3, beta, Recombinant, Human (Tyrosine 3-monooxygenase/tryptopan 5-monooxygenase activation protein, beta polypeptide)
100ug
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Technical Data |
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0014-02D |
14-3-3, beta, Recombinant, Human (Tyrosine 3-monooxygenase/tryptopan 5-monooxygenase activation protein, beta polypeptide) |
100ug |
| Molecular Biology | Storage: -20°CShipping: Dry Ice |
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The 14-3-3 family plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 beta, a subtype of the 14-3-3 proteins, was found in B Cells, brain and liver etc. This 14-3-3 beta has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Source: Synthetic corresponding to aa1-246 of human 14-3-3 beta expressed in E .coli. AA Sequences: MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL ELLDKYLIPN ATQPESKVFY LKMKGDYFRY LSEVASGDNK QTTVSNSQQA YQEAFEISKK EMQPTHPIRL GLALNFSVFY YEILNSPEKA CSLAKTAFDE AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD AGEGEN Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 28kD |
Source: Synthetic corresponding to aa1-246 of human 14-3-3 beta expressed in E .coli. Purity: 90% by SDS-PAGE Concentration: ~1mg/ml Form: Supplied as a liquid in 20mM Tris pH 8.0, 1mM EDTA, 50mM NaCl. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Rodriguez LG., et al. (2005). J Cell Physiol. Jan; 202(1):285-94. 2. Mils V., et al.(2000). Oncogene. Mar 2; 19(10):1257-65.
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