Technical Data
0014-02G
14-3-3 beta, alpha, CT (Protein 1054, Protein Kinase C Inhibitor Protein 1, brain protein 14-3-3, beta Isoform, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide, protein kinase C inhibitor protein-1)
Description:
Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer's. The 14-3-3 beta antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer's and Parkinson's Disease.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:40
Western Blot: 1:1000
Immunohistochemistry (paraffin): 1:500
Immunocytochemistry: 1:500
Immunoprecipitation: 1:40
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cell lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9i142Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to C-terminus residues of human 14-3-3 beta, alpha.
Purity:
Supernatant
Form
Supplied as a liquid in Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human 14-3-3 beta, alpha. Species Crossreactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fu, H., et al. (2000). Ann. Rev. Pharmacol. Toxicol. 40: 617647. 2. Muslin, A., et al. (1996). Cell 84: 889897. 3. Zha, J., et al. (1996). Cell 87: 619628.