Technical Data
0014-02L-HRP
14-3-3 beta, alpha, CT (Protein 1054, Protein Kinase C Inhibitor Protein 1, KCIP-1, 14-3-3 Protein beta/alpha, N-terminally Processed, YWHAB) (Azide free) (HRP)
Description:
YWHAB belongs to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Note: Applications are based on unconjugated antibody.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul4C Do Not FreezeBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the C-terminal region of human YWHAB (KLH).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Conjugated to HRP.
Specificity:
Recognizes human YWHAB.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Komori, T., et al., Acta Neuropathol. 106(1):66-70 (2003). 2.Cavet, M.E., et al., J. Biol. Chem. 278(20):18376-18383 (2003). 3.Li, Y., et al., J. Biol. Chem. 278(16):13663-13671 (2003). 4.Shumway, S.D., et al., J. Biol. Chem. 278(4):2089-2092 (2003). 5.Parvaresch, S., et al., FEBS Lett. 532(3):357-362 (2002).