Technical Data
0014-02P1
14-3-3 beta (Tyrosine 3-monooxygenase/tryptopan 5-monooxygenase Activation Protein beta Polypeptide, YWHAB)
Description:
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.

Applications:
Suitable for use in Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5000-1:20,000
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Immunofluorescence: 1:100-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to aa1-141 of human 14-3-3 beta.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7, 0.01% thimerosal, 10% glycerol.
Specificity:
Recognizes human YWHAB. Species sequence homology: mouse (85%), rat (97%), porcine (100%) and zebrafish (84%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.