Technical Data
14-3-3, beta, Recombinant, Human (Protein 1054, Protein Kinase C Inhibitor Protein 1, KCIP-1, 14-3-3 Protein beta/alpha, N-terminally Processed, YWHAB)
Molecular Biology Storage: -20CShipping: Blue Ice
14-3-3, a family of acidic and soluble proteins, highly conserved in aa sequences from yeast to mammals, is expressed in all eukaryotic cells. Seven isoforms (beta, gamma, epsilon, eta, zeta, sigma and tau/theta) encoded by seven distinct genes are identified in mammals and forms homo- and hetero- dimeric cup-shaped structures. As 14-3-3 is interacted with more than 100 binding partners, it regulates key proteins involved in various biological processes such as signal transduction, cell cycle, transcriptional control, cell proliferation, apoptosis, and ion channel physiology. Most 14-3-3 requires phosphorylation of serine or threonine residues in the target sequence. This protein is abundantly expressed in the brain and has been detected in the cerebrospinal fluid of patients with different neurological disorders.

Recombinant corresponding to aa1-246, with additional 3aa-GSH at N-term, from human 14-3-3b, expressed in E.coli.

Molecular Weight:

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E.coli
Purity: ~95% (SDS-PAGE)
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder from 20mM Tris, pH 8.0, 10mM sodium chloride. Reconstitute with sterile dH2O PBS.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Tzivion, G. et al. (2001) Oncogene, 20, 6331-6338. 2. Tzivion, G. and Avruch, J. (2002) J.Biol.Chem. 277, 3061-3064. 3. Berg, D. et al. (2003) Nat. Rev. Neurosci. 4(9), 752-762.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.