Technical Data
0014-03D
14-3-3 beta, zeta
Description:
14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exist in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:50
Western Blot: 1ug/ml. Predicted MW 29 and 30kD.
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa Whole Cell Extract

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG9A69Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Lysed transformed human amnion cells (AMA) . Cellular Localization: Cytoplasmic.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1mM PMSF, 50% glycerol.
Specificity:
Recognizes human 14-3-3 beta, zeta. Species Crossreactivity: mouse, rat, bovine, canine, monkey, porcine, rabbit and sheep.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Choi EK et al. Calsenilin is a substrate for caspase-3 that preferentially interacts with the familial Alzheimer's disease-associated C-terminal fragment of presenilin 2. J Biol Chem 276:19197-204 (2001). 2. Celis A et al. Human proteins IEF 58 and 57a are associated with the Golgi apparatus. FEBS Lett 227:14-20 (1988). 3. Aitken A et al. 14-3-3 proteins: biological function and domain structure. Biochem Soc Trans 23:605-11 (1995). 4. Burbelo PD & Hall A 14-3-3 proteins. Hot numbers in signal transduction. Curr Biol 5:95-6 (1995).