Technical Data
0014-04D
14-3-3, 30, 33kD (Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Polypeptide, 14-3-3 epsilon, Mitochondrial Import Stimulation Factor L, Protein Kinase C Inhibitor Protein 1, YWHAE)
Description:
Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer's.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat cell lysates.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceRatRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to rat 14-3-3 epsilon, amino acids 208-244.
Purity:
Serum
Form
Supplied as a liquid. No preservative added.
Specificity:
Recognizes human 14-3-3 proteins. Species Crossreactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.