		Technical Data
0014-04E	14-3-3 Protein epsilon, phosphorylated (Ser58) (YWHAE, 14-3-3E)

Description:
The 14-3-3 family of proteins mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
200ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser58 of human 14-3-3 (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human 14-3-3 when phosphorylated at Ser58.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Urschel, S., et al., J. Biol. Chem. 280(17):16987-16993 (2005). 2. Yanagi, M., et al., J. Hum. Genet. 50(4):210-216 (2005). 3. Wang, X., et al., J. Biol. Chem. 279(47):49460-49469 (2004). 4. Clark, K.L., et al., J. Biol. Chem. 279(19):19401-19406 (2004). 5. Wang, X., et al., Mol. Cell. Biol. 23(17):6013-6026 (2003).