Technical Data
0014-07A
14-3-3, sigma (SFN, Stratifin, HGNC:10773) (BSA & Azide Free)
Description:
14-3-3 proteins are 30kD polypeptides with nine closely related members in mammals. They are also found in plants and fungi. 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins which bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 proteins form homo- and heterodimeric cup-like structures that bind to discrete phosphoserine-containing motifs. In some instances, 14-3-3 proteins appear to export their binding partners from the nucleus to the cytoplasm in a phosphorylation and Crm1-dependent manner. 14-3-3 sigma shares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130, p21Cip1, p27Kip1 and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and in vivo. Expression of 14-3-3 sigma is higher in normal epithelial cells.

Applications:
Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunoprecipitation: 2ug/mg protein lysate. Use Protein G.
Western Blot: 1ug/ml for 2hrs at RT.
Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
HT29 cells, Skin

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k1.N.6Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant human 14-3-3 sigma protein (26-30kD). Cellular Localization: Cytoplasmic.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. No stabilizing proteins and no preservatives added. Also supplied with BSA and sodium azide. See Cat# 0014-19A.
Specificity:
Recognizes human 14-3-3 sigma protein (26-30kD). Does not crossreact with other isoforms of 14-3-3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Laronga, C., et al., J. Biol. Chem. 275: 23,106-23,112 (2000). 2. Villaret, D.B., et al., Laryngoscope 110: 374-381 (2000). 3. Hermeking, H., et al., Mol. Cell 1: 3-11 (1997).