Technical Data
0014-07F
14-3-3, gamma, Recombinant, Human (Protein Kinase C Inhibitor Protein 1, KCIP-1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase Activation Protein, gamma Polypeptide, YWHAG)
100ug
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Technical Data |
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0014-07F |
14-3-3, gamma, Recombinant, Human (Protein Kinase C Inhibitor Protein 1, KCIP-1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase Activation Protein, gamma Polypeptide, YWHAG) |
100ug |
| Molecular Biology | Storage: -20°CShipping: Blue Ice |
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The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms B, Y, E, O, Z, T, and n proteins that have been identified in mammals. The 14-3-3gamma, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. Recombinant human 14-3-3 Y was expressed in E. coli and purified by using conventional chromatography techniques. Source: Synthetic corresponding to aa1-247 of human 14-3-3, gamma, expressed in E.coli. AA Sequence: MVDREQLVQK ARLAEQAERY DDMAAAMKNV TELNEPLSNE ERNLLSVAYK NVVGARRSSW RVISSIEQKT SADGNEKKIE MVRAYREKIE KELEAVCQDV LSLLDNYLIK NCSETQYESK VFYLKMKGDY YRYLAEVATG EKRATVVESS EKAYSEAHEI SKEHMQPTHP IRLGLALNYS VFYYEIQNAP EQACHLAKTA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDD DGGEGNN Endotoxin: 1.0 EU per 1ug of protein (determined by LAL method) Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 28.3kD |
Source: Recombinant, Human, His- tag, E. coli Purity: 95% by SDS-PAGE Concentration: ~1mg/ml Form: Supplied as a liquid in 20mM Tris, pH 7.5 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Chen, X.Q., et al., Glia 42(4): 315-324 (2005). 2. Chen, X.Q. & Yu, A.C., et al., J. Biochem. 296(3): 657-663 (2002).
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