Technical Data
0014-07F
14-3-3, gamma, Recombinant, Human (Protein Kinase C Inhibitor Protein 1, KCIP-1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase Activation Protein, gamma Polypeptide, YWHAG)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint
control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms B, Y, E, O, Z, T, and n proteins that have been identified in mammals. The 14-3-3gamma, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. Recombinant human 14-3-3 Y was expressed in E. coli and purified by using conventional chromatography techniques.

Source:
Synthetic corresponding to aa1-247 of human 14-3-3, gamma, expressed in E.coli.

AA Sequence:
MVDREQLVQK ARLAEQAERY DDMAAAMKNV TELNEPLSNE ERNLLSVAYK NVVGARRSSW RVISSIEQKT SADGNEKKIE MVRAYREKIE KELEAVCQDV LSLLDNYLIK NCSETQYESK VFYLKMKGDY YRYLAEVATG EKRATVVESS EKAYSEAHEI SKEHMQPTHP IRLGLALNYS VFYYEIQNAP EQACHLAKTA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDD DGGEGNN

Endotoxin:
1.0 EU per 1ug of protein (determined by LAL method)

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
28.3kD
Source: Recombinant, Human, His- tag, E. coli
Purity: 95% by SDS-PAGE
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20mM Tris, pH 7.5

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Chen, X.Q., et al., Glia 42(4): 315-324 (2005). 2. Chen, X.Q. & Yu, A.C., et al., J. Biochem. 296(3): 657-663 (2002).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.