Technical Data
0014-07K
14-3-3 gamma (Protein Kinase C Inhibitor Protein 1, KCIP-1, YWHAG)
Description:
14.3.3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14.3.3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14.3.3 and target proteins are strongly influenced by the phosphorylation state of 14.3.3 and the target protein. Expression of 14.3.3-g is higher in cells from vascular smooth muscle, skeletal and heart muscle. In Alzheimer's disease and Down syndrome level of 14.3.3-g in brain increases significantly.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml for 2h at RT
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k2D9Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Recombinant corresponding to human 14-3-3 gamma.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Specificity:
Recognizes human 14-3-3 gamma.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jackson, W. et al. Plos Bio. 3, e156 (2005). 2. He, H. et al. J Biol Chem 11, 11 (2003). 3. Zhou, B. et al. J Clin Invest 100, 3070 (1997).