		Technical Data
0014-07K	14-3-3 gamma (Protein Kinase C Inhibitor Protein 1, KCIP-1, YWHAG)

Description:
14.3.3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14.3.3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14.3.3 and target proteins are strongly influenced by the phosphorylation state of 14.3.3 and the target protein. Expression of 14.3.3-g is higher in cells from vascular smooth muscle, skeletal and heart muscle. In Alzheimer's disease and Down syndrome level of 14.3.3-g in brain increases significantly.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml for 2h at RT
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	2D9	Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Recombinant corresponding to human 14-3-3 gamma.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.

Specificity:

Recognizes human 14-3-3 gamma.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Jackson, W. et al. Plos Bio. 3, e156 (2005). 2. He, H. et al. J Biol Chem 11, 11 (2003). 3. Zhou, B. et al. J Clin Invest 100, 3070 (1997).