Technical Data
0014-07M
14-3-3 gamma, Recombinant, Human, Western Blot Positive Control (Protein Kinase C Inhibitor Protein 1, KCIP-1, 14-3-3 Protein gamma, N-terminally Processed, YWHAG)
10ug
Molecular Biology Storage: -20CShipping: Blue Ice
14-3-3 is a general name given to a family of acidic, soluble, ubiquitously expressed 28kD proteins that are the products of related but separate genes. They are considered isoforms and are named eta, gamma, delta/zeta, alpha/beta, theta/tau, sigma or epsilon. Interactions are generally mediated by phosphorylation of specific binding sites in the target proteins. 14-3-3 proteins can exist as monomers, homodimers and heterodimers.

Source:
Recombinant corresponding to aa2-247 from human 14-3-3-Gamma, fused to N-terminal 6x His-tag, expressed in E. coli.

Molecular Weight:
~28kD

Applications:
Suitable for use in Western Blot as a control. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~28kD
Source: E. coli
Purity: Purified (SDS-PAGE)
Concentration: As reported
Form: Supplied as a liquid in 25mM Tris-HCl, pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue, 2.5% glycerol.
Specificity:


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Mhawech P. (2005) Cell Research 15 (4); 228-236.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.