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Technical Data |
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0014-07N |
14-3-3 gamma, Recombinant, Human (Protein Kinase C Inhibitor Protein 1, KCIP-1, 14-3-3 Protein gamma, N-terminally Processed, YWHAG) |
10ug 100ug |
| Molecular Biology | Storage: -20°CShipping: Blue Ice |
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14-3-3 is a general name given to a family of acidic, soluble, ubiquitously expressed 28kD proteins that are the products of related but separate genes. They are considered isoforms and are named eta, gamma, delta/zeta, alpha/beta, theta/tau, sigma or epsilon. Interactions are generally mediated by phosphorylation of specific binding sites in the target proteins. 14-3-3 proteins can exist as monomers, homodimers and heterodimers. Source: Recombinant corresponding to aa2-247 from human 14-3-3 gamma, fused to N-terminal 6x His-tag, expressed in E. coli. Molecular Weight: ~28kD Applications: Suitable for use in ELISA, Western Blot, Dot Blot, Immunohistochemistry, Immunoassays and in the development of rapid tests and other immunoassays. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: ~28kD |
Source: E. coli Purity: ~95% (SDS-PAGE) Concentration: As reported Form: Supplied as a liquid in saline or tris, 50% glycerol. BSA free. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Mhawech P. (2005) Cell Research 15(4); 228-236.
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