Technical Data
0014-19D
14-3-3 sigma (Epithelial Cell Marker Protein 1, Stratifin, SFN, HME1)
Description:
14.3.3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14.3.3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14.3.3 and target proteins are strongly influenced by the phosphorylation state of 14.3.3 and the target protein. 14.3.3-sigma shares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130, p21Cip1, p27Kip1, and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and in vivo.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml for 2hrs at RT
Immunoprecipitation: 2ug/mg protein lysate
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k2D10Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Recombinant corresponding to human 14.3.3 sigma.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Specificity:
Recognizes human 14.3.3 sigma.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.