Technical Data
0014-20
14-3-3, tau (YWHAQ, 1C5, HS1, Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide), Recombinant, Human
50ug
100ug
100ug
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Technical Data |
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0014-20 |
14-3-3, tau (YWHAQ, 1C5, HS1, Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide), Recombinant, Human |
50ug 100ug |
| Molecular Biology | Storage: -20°CShipping: Dry Ice |
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The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms that have been identified in mammals. The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes. This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis. Recombinant human YWHAQ was expressed in E. coli and purified by using conventional chromatography techniques. Source: Synthetic corresponding to aa1-245 of human 14-3-3, tau, expressed in E.coli. AA Sequences: MEKTELIQKA KLAEQAERYD DMATCMKAVT EQGAELSNEE RNLLSVAYKN VVGGRRSAWR VISSIEQKTD TSDKKLQLIK DYREKVESEL RSICTTVLEL LDKYLIANAT NPESKVFYLK MKGDYFRYLA EVACGDDRKQ TIDNSQGAYQ EAFDISKKEM QPTHPIRLGL ALNFSVFYYE ILNNPELACT LAKTAFDEAI AELDTLNEDS YKDSTLIMQL LRDNLTLWTS DSAGEECDAA EGAEN Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 27kD |
Source: Synthetic corresponding to aa1-245 of human 14-3-3, tau, expressed in E. coli. Purity: ~95% by SDS-PAGE. Purified by chromatography techniques. Concentration: ~1mg/ml Form: Supplied as a liquid in 20mM Tris pH7.5 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Liu YC , et al. (1996) J Biol Chem; 271: 14591-5 2. Xiao B , et al. (1995) Nature, 376; 188-191
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