		Technical Data
0014-21	14-3-3 theta (14-3-3 tau)

Description:
The 14-3-3 proteins are a family of homologous, evolutionarily conserved, acidic proteins expressed in all eukaryotes, with molecular masses ranging from 28-33kD. To date, seven different 14-3-3 isoforms have been identified in mammals, each encoded by a distinct gene. The expression of 14-3-3 isoforms is tissue specific and differentially regulated. The 14-3-3 proteins assemble into stable homo- and heterodimers, which modulate signaling cascades by binding to phosphorylated serine or threonine residues of target proteins. In addition to a phosphorylated serine or phohsphorylated threonine residue, 14-3-3 protein binding also requires an arginine located at position –3 or –4. The 14-3-3 proteins affect signaling in several manners, including: interfering with protein/protein interactions (example: 14-3-3’s interference
with the BAD/Bcl-2 interaction, inhibiting apoptosis); modulating target nuclear transport (examples: 14-3-3’s modulation of nuclear transport of Cdc25, telomerase and forkhead transcription factors); modulating target catalytic activity (example: 14-3-3’s binding to tryptophan hydrolase, enhancing its catalytic activity); and protecting targets from proteolyis (examples: 14-3-3’s protection of Raf and BAD). The 14-3-3 proteins also modulate signaling cascades by serving as scaffolding/adapter proteins. The measurement of 14-3-3 isoforms in cerebrospinal fluid has utility in diagnosing prion diseases such as Creutzfeldt-Jakob disease.

Applications:
Suitable for use in Western Blot and Immunoprecipitation of both the native and denatured forms of the protein.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Positive Controls:
PC12 cells, 3T3 cells, MCBK cells, Jurkat cells, or normal human fibroblasts.

Hybridoma:
Balb/c splenocytes

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	5J20	Purified

Size		Storage	Shipping	Source	Host
50ug		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Recombinant 14-3-3 theta/tau (a single protein which is known by the alternative names 14-3-3 theta and 14-3-3 tau).

Purity:

Purified

Form

Supplied as a liquid in 20mM sodium phosphate, 150mM sodium chloride, pH 7.5, 0.08% sodium azide, 50% glycerol.

Specificity:

Recognizes human 14-3-3 theta/tau. Does not crossreact with 14-3-3 zeta. 14-3-3 theta/tau is expressed by brain tissue and cells of the male germ line. Species Crossreactivity: mouse, rat and bovine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Tzivion, G. and Avruch, J., J. Biol. Chem. 277: 3061-3064 (2002). 2. Lee, K.H. and M.G Harrington, M.G., Electrophoresis 18(3-4): 502-506 (1997). 3. Perego, L. and Berruti, G., Mol. Reprod. Dev. 47(3): 370-379 (1997). 4. Sanchez-Valle, R., et al., Neurosci. Lett. 320(1-2): 69-72 (2002). 5. Subramanian, R.R., et al., Exp. Cell Res. 271(1): 142-151 (2001). 6. Wiltfang, J., et al., J. Neurochem. 73(6): 2485-2490 (1999).