Technical Data
0014-21
14-3-3 theta (14-3-3 tau)
Description:
The 14-3-3 proteins are a family of homologous, evolutionarily conserved, acidic proteins expressed in all eukaryotes, with molecular masses ranging from 28-33kD. To date, seven different 14-3-3 isoforms have been identified in mammals, each encoded by a distinct gene. The expression of 14-3-3 isoforms is tissue specific and differentially regulated. The 14-3-3 proteins assemble into stable homo- and heterodimers, which modulate signaling cascades by binding to phosphorylated serine or threonine residues of target proteins. In addition to a phosphorylated serine or phohsphorylated threonine residue, 14-3-3 protein binding also requires an arginine located at position 3 or 4. The 14-3-3 proteins affect signaling in several manners, including: interfering with protein/protein interactions (example: 14-3-3s interference
with the BAD/Bcl-2 interaction, inhibiting apoptosis); modulating target nuclear transport (examples: 14-3-3s modulation of nuclear transport of Cdc25, telomerase and forkhead transcription factors); modulating target catalytic activity (example: 14-3-3s binding to tryptophan hydrolase, enhancing its catalytic activity); and protecting targets from proteolyis (examples: 14-3-3s protection of Raf and BAD). The 14-3-3 proteins also modulate signaling cascades by serving as scaffolding/adapter proteins. The measurement of 14-3-3 isoforms in cerebrospinal fluid has utility in diagnosing prion diseases such as Creutzfeldt-Jakob disease.

Applications:
Suitable for use in Western Blot and Immunoprecipitation of both the native and denatured forms of the protein.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Positive Controls:
PC12 cells, 3T3 cells, MCBK cells, Jurkat cells, or normal human fibroblasts.

Hybridoma:
Balb/c splenocytes

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15J20Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant 14-3-3 theta/tau (a single protein which is known by the alternative names 14-3-3 theta and 14-3-3 tau).
Purity:
Purified
Form
Supplied as a liquid in 20mM sodium phosphate, 150mM sodium chloride, pH 7.5, 0.08% sodium azide, 50% glycerol.
Specificity:
Recognizes human 14-3-3 theta/tau. Does not crossreact with 14-3-3 zeta. 14-3-3 theta/tau is expressed by brain tissue and cells of the male germ line. Species Crossreactivity: mouse, rat and bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tzivion, G. and Avruch, J., J. Biol. Chem. 277: 3061-3064 (2002). 2. Lee, K.H. and M.G Harrington, M.G., Electrophoresis 18(3-4): 502-506 (1997). 3. Perego, L. and Berruti, G., Mol. Reprod. Dev. 47(3): 370-379 (1997). 4. Sanchez-Valle, R., et al., Neurosci. Lett. 320(1-2): 69-72 (2002). 5. Subramanian, R.R., et al., Exp. Cell Res. 271(1): 142-151 (2001). 6. Wiltfang, J., et al., J. Neurochem. 73(6): 2485-2490 (1999).