Technical Data
0014-22A
14-3-3 zeta, Phosphorylated (Ser58)
Description:
Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:100-1:200
Western Blot: 1:500-1:1,000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthesized phosphopeptide derived from human 14-3-3 around the phosphorylation site of serine 58 (R-S-SP-W-R).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes endogenous levels of 14-3-3 only when phosphorylated at serine 58.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.