Technical Data
0014-22B
14-3-3 zeta (MGC111427, MGC126532, MGC138156, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase Activation Protein zeta Polypeptide, YWHAZ, YWHAZ Protein)
Description:
Applications:
Suitable for use in ELISA, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul4C/-20C (No Glycerol)Blue IceSheepRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide, Ac.MDKNELVQKAC, corresponding to acetylated Nterminal sequence of sheep 1433 zeta.
Purity:
Serum
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes sheep 1433 at ~30kD. Species Crossreactivity: bovine, chicken, rat, human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Martin, H. et al. (1993) Antibodies against major brain isoforms of 1433 protein. An antibody specific for the Nacetylated aminoterminus of a protein. FEBS 331: 296303. 2. Aitken, A. et al. (2006) 1433
proteins: A historic overview. Seminars in Cancer Biology 16: 162172.