Technical Data
0014-22E
14-3-3 zeta (14-3-3 Protein zeta/delta, Protein Kinase C Inhibitor Protein 1, KCIP-1, YWHAZ)
Description:
14-3-3 z is a member of a conserved family of proteins that interact with hundreds of different proteins and thus mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 z is localized mostly on dense granules and is secreted after platelet activation. The protein is highly abundant in brain. The protein act as an activators of tyrosine and tryptophan hydroxylases. Human 14-3-3 z gene is located on chromosome 8q23.1, which is close to a probable bipolar disorder susceptibility locus 8q24.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.

Positive Control:
NIH/3T3 cells

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic non-phosphopeptide corresponding to human 14-3-3 z around the phosphorylation site of serine 58 (R-S-SP-W-R).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human 14-3-3 zeta. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yanbin. J. et al. European Psychiatry. 19:377-379 (2004).