		Technical Data
0014-22G	14-3-3 zeta (14-3-3 Protein zeta/delta, Protein Kinase C Inhibitor Protein 1, KCIP-1, YWHAZ)

Description:
14-3-3 zeta is a 245aa containing protein belonging to the 14-3-3 family mediating signal transduction by binding to phosphoserine containing proteins. It is a highly conserved protein in both plants and mammals. The encoded protein interacts with IRS1 implicating a plausible role in regulating insulin sensitivity. There are several transcript variants, with seven known mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta and eta. 14-3-3 proteins function in broad regulation of proteins by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, and activation/repression of enzyme activity. The protein is highly abundant in brain.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Brain

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to aa200-245 of human 14-3-3 zeta.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

Specificity:

Recognizes human 14-3-3 zeta. Species Crossreactivity: chimpanzee, bovine, canine, mouse, rat, rhesus monkey. Species sequence homology: xenopus.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Murthi, P. et al. Placenta 29:798-801 (2008). 2. Frasor, J. et al. Cancer Res. 66:7334-7340 (2006). 3. Heidenblad, M. et al. BMC Med. Genomics 31:3 (2008).