Technical Data
0014-24B
14-3-3 zeta/delta (T232) (14-3-3 Protein zeta/delta, Protein Kinase C Inhibitor Protein 1, KCIP-1, YWHAZ)
Description:
14-3-3 protein zeta/delta belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to amino acid residues surrounding T232 of human 14-3-3 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human 14-3-3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Li,F.Q., J. Cell Biol. 181(7), 1141-1154 (2008). 2.Mateo,I., Eur. J. Neurol. 15(3), 219-222 (2008). 3.Li,Z., Proc. Natl. Acad. Sci. USA 105(1), 162-167 (2008). 4.Powell,D.W., Mol. Cell. Biol. 23(15), 5376-5387 (2003). 5.Powell,D.W., J. Biol. Chem. 277(24), 21639-21642 (2002).