		Technical Data
0014-24B	14-3-3 zeta/delta (T232) (14-3-3 Protein zeta/delta, Protein Kinase C Inhibitor Protein 1, KCIP-1, YWHAZ)

Description:
14-3-3 protein zeta/delta belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide corresponding to amino acid residues surrounding T232 of human 14-3-3 (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human 14-3-3.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Li,F.Q., J. Cell Biol. 181(7), 1141-1154 (2008). 2.Mateo,I., Eur. J. Neurol. 15(3), 219-222 (2008). 3.Li,Z., Proc. Natl. Acad. Sci. USA 105(1), 162-167 (2008). 4.Powell,D.W., Mol. Cell. Biol. 23(15), 5376-5387 (2003). 5.Powell,D.W., J. Biol. Chem. 277(24), 21639-21642 (2002).