Technical Data
022892-01
WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monosodium salt)
10mg
30mg
Biochemicals Storage: -20CShipping: Blue Ice
A next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH. It is reduced by NADH at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460nM. Useful in cell proliferation assays as a cell viability indicator.

Sequence:
2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monosodium salt

Molecular Weight:
~600.47

Solubility:
H2O (~100mM)

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with H2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

CAS Number:
193149-74-5

Molecular Formula:
C20H13N6O11S2Na

Molecular Weight:
600.47
Purity: ~97% (HPLC)
Concentration: Not determined
Form: Supplied as a pale brown solid.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.