Technical Data
028975
Acrp30 (30kD Adipocyte Complement-related Protein, ACDC, Adipocyte C1q and Collagen Domain Containing Protein, Adiponectin, ADPN, ADIPOQ, Adipose Most Abundant Gene Transcript 1 Protein, ADIPQTL1, APM1, apM-1, Gelatin-binding Protein, GBP28) (HRP)
Description:
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouseRabbit
Concentration:
As reported
Immunogen:
Recombinant corresponding to mouse Adiponectin.
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Specificity:
Recognizes mouse Acrp30.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. "A novel serum protein similar to C1q, produced exclusively in adipocytes."Scherer P.E., Williams S., Fogliano M., Baldini G., Lodish H.F.J. Biol. Chem. 270:26746-26749 (1995). 2. "AdipoQ is a novel adipose-specific gene dysregulated in obesity."Hu E., Liang P., Spiegelman B.M.J. Biol. Chem. 271:10697-10703 (1996). 3. "Chromosomal localization, expression pattern, and promoter analysis of the mouse gene encoding adipocyte-specific secretory protein Acrp30." Das K., Lin Y., Widen E., Zhang Y., Scherer P.E. Biochem. Biophys. Res. Commun. 280:1120-1129 (2001).