Technical Data
029032
ARF4 (ADP-ribosylation Factor 4, ARF2)
Description:
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Recombinant corresponding to human ADP-ribosylation factor 4.
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity:
Recognizes human ARF4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. "Selective amplification of an mRNA and related pseudogene for a human ADP-ribosylation factor, a guanine nucleotide-dependent protein activator of cholera toxin." Monaco L., Murtagh J.J. Jr., Newman K.B., Tsai S.-C., Moss J., Vaughan M. Proc. Natl. Acad. Sci. U.S.A. 87:2206-2210 (1990). 2. "Human ADP-ribosylation factors. A functionally conserved family of GTP-binding proteins." Kahn R.A., Kern F.G., Clark J., Gelmann E.P., Rulka C. J. Biol. Chem. 266:2606-2614 (1991). 3. "Cloning and characterization of the human ADP-ribosylation factor 4 gene." Lebeda R.A., Haun R.S. Gene 237:209-214 (1999). 4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S.