		Technical Data
029035	ARF4 (ADP-ribosylation Factor 4, ARF2) (HRP)

Description:
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Recombinant corresponding to human ADP-ribosylation factor 4.

Purity:

Purified by ammonium sulfate precipitation.

Form

Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP).

Specificity:

Recognizes human ARF4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. "Selective amplification of an mRNA and related pseudogene for a human ADP-ribosylation factor, a guanine nucleotide-dependent protein activator of cholera toxin." Monaco L., Murtagh J.J. Jr., Newman K.B., Tsai S.-C., Moss J., Vaughan M. Proc. Natl. Acad. Sci. U.S.A. 87:2206-2210 (1990). 2. "Human ADP-ribosylation factors. A functionally conserved family of GTP-binding proteins." Kahn R.A., Kern F.G., Clark J., Gelmann E.P., Rulka C. J. Biol. Chem. 266:2606-2614 (1991). 3. "Cloning and characterization of the human ADP-ribosylation factor 4 gene." Lebeda R.A., Haun R.S. Gene 237:209-214 (1999). 4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S.