		Technical Data
029065	ATP6V1E1 (V-type Proton ATPase Subunit E 1, V-ATPase Subunit E 1, V-ATPase 31kD Subunit, p31, Vacuolar Proton Pump Subunit E 1, ATP6E, ATP6E2) (HRP)

Description:
Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Recombinant corresponding to human ATP6V1E1.

Purity:

Purified by ammonium sulfate precipitation.

Form

Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP).

Specificity:

Recognizes human ATP6V1E1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. "Immunologic evidence that vacuolar H+ ATPases with heterogeneous forms of Mr = 31,000 subunit have different membrane distributions in mammalian kidney."Hemken P., Guo X.-L., Wang Z.-Q., Zhang K., Gluck S.J. Biol. Chem. 267:9948-9957 (1992). 2. "The E subunit of vacuolar H(+)-ATPase localizes close to the centromere on human chromosome 22."Baud V., Mears A., Lamour V., Scamps C., McDermid A., Lipinski M.Hum. Mol. Genet. 3:335-339 (1994). 3. "Cloning and tissue distribution of subunits C, D, and E of the human vacuolar H(+)-ATPase." van Hille B., Vanek M., Richener H., Green J.R., Bilbe G.Biochem. Biophys. Res. Commun. 197:15-21 (1993).