		Technical Data
029145	ENSA (Alpha-endosulfine, ARPP-19e) (HRP)

Description:
Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase.Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Recombinant corresponding to human Alpha-endosulfine.

Purity:

Purified by ammonium sulfate precipitation.

Form

Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP).

Specificity:

Recognizes human ENSA.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. "Human alpha-endosulfine, a possible regulator of sulfonylurea-sensitive K(ATP) channel: molecular cloning, expression and biological properties." Heron L., Virsolvy A., Peyrollier K., Gribble F.M., Le Cam A., Ashcroft F.M., Bataille D.Proc. Natl. Acad. Sci. U.S.A. 95:8387-8391 (1998). 2. "Isolation, characterization, and chromosomal localization of the human ENSA gene that encodes alpha-endosulfine, a regulator of beta-cell K(ATP) channels."Heron L., Virsolvy A., Apiou F., Le Cam A., Bataille D.Diabetes 48:1873-1876 (1999). 3. "The transcribed endosulfine alpha gene is located within a type 2 diabetes-linked region on 1q: sequence and expression analysis in Pima Indians." Thameem F., Farook V.S., Yang X., Lee Y.-H., Permana P.A., Bogardus C., Prochazka M.Mol. Genet. Metab. 81:16-21 (2004).