Technical Data
P4HB (Protein Disulfide-isomerase, PDI, Cellular Thyroid Hormone-binding Protein, Prolyl 4-hydroxylase Subunit beta, p55, ERBA2L, PDI, PDIA1, PO4DB) (HRP)
Dsb proteins (DsbA, DsbB, DsbC, and DsbD) catalyze formation and isomerization of protein disulfide bonds in the periplasm of Escherichia coli. Disulphide bond isomerase (DsbC), a member of the thioredoxin superfamily, acts as a disulfide isomerase and converts aberrant disulfide bonds to correct ones. It also acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbC is reoxidized by a yet uncharacterized protein.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
100ug-20CBlue IceHumanRabbit
As reported
Recombinant corresponding to human disulfide-isomerase.
Purified by ammonium sulfate precipitation.
Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Recognizes human P4HB.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. "Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene." Pihlajaniemi T., Helaakoski T., Tasanen K., Myllylae R., Huhtala M.-L., Koivu J., Kivirikko K.I. EMBO J. 6:643-649 (1987). 2. "The nucleotide sequence of a human cellular thyroid hormone binding protein present in endoplasmic reticulum." Cheng S.-Y., Gong Q.-H., Parkison C., Robinson E.A., Appella E., Merlino G.T., Pastan I. J. Biol. Chem. 262:11221-11227 (1987). 3. "Characterization of the human gene for a polypeptide that acts both as the beta subunit of prolyl 4-hydroxylase and as protein disulfide isomerase." Tasanen K., Parkkonen T., Chow L.T., Kivirikko K.I., Pihlajaniemi T. J. Biol. Chem. 263:16218-16224 (1988).