Technical Data
029294
RHOA (Transforming Protein RhoA, Rho cDNA Clone 12, ARH12, ARHA, RHO12) (FITC)
Description:
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Recombinant corresponding to human RhoA.
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes human RhoA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. "Nucleotide sequence of human rho cDNA clone 12." Yeramian P., Chardin P., Madaule P., Tavitian A. Nucleic Acids Res. 15:1869-1869 (1987). 2. "Sequence of rho small GTP-binding protein cDNAs from human retina and identification of novel 5' end cloning artifacts." Fagan K.P., Oliveira L., Pittler S.J. Exp. Eye Res. 59:235-237 (1994). 3. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. 4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.