Technical Data
Thioredoxin (Trx, ATL-derived Factor, ADF, Surface-associated Sulphydryl Protein, SASP, TXN, TRDX, TRX, TRX1)
Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
50ug-20CBlue IceHumanRabbit
As reported
Recombinant corresponding to human Thioredoxin.
Purified by ammonium sulfate precipitation.
Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Recognizes human Thioredoxin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. "Nucleotide sequence of a cDNA encoding rat thioredoxin."Tonissen K.F., Robins A.J., Wells J.R.E. Nucleic Acids Res. 17:3973-3973 (1989). 2. Xie Z.H., Liu C.Z., He Y.H., Wang A.M., Ma C. 3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project TeamGenome Res. 14:2121-2127 (2004).