		Technical Data
029384	Thioredoxin (Trx, ATL-derived Factor, ADF, Surface-associated Sulphydryl Protein, SASP, TXN, TRDX, TRX, TRX1) (FITC)

Description:
Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Recombinant corresponding to human Thioredoxin.

Purity:

Purified by ammonium sulfate precipitation.

Form

Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with fluorescein isothiocyanate (FITC).

Specificity:

Recognizes human Thioredoxin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. "Nucleotide sequence of a cDNA encoding rat thioredoxin."Tonissen K.F., Robins A.J., Wells J.R.E. Nucleic Acids Res. 17:3973-3973 (1989). 2. Xie Z.H., Liu C.Z., He Y.H., Wang A.M., Ma C. 3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project TeamGenome Res. 14:2121-2127 (2004).