Technical Data
5-Methylcytosine (5mC, 5-Methyl-Cytosine, 5 MethylCytidine, 5 MeCyd, 5 Me Cytidine)
5-Methylcytosine is a modified base found in the DNA of higher eukaryotes. Methylation of DNA is known to be involved in the control of gene expression and is mediated by a class of enzymes known as DNA methyltransferases (DNMT's). Appropriate cytosine methylation is essential for normal development and maintenance of mammalian cells. Alternations in DNA methylation patterns is often associated with cancers with the turmor DNA being hypomethylated compared to DNA from normal tissues.

Suitable for use in Dot Blot. Other applications not tested.

Recommended Dilution:
Dot Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Methylated and unmethylated BRCA1

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1,l13A149Affinity Purified
100ug-20CBlue IceHumanMouse
5-methylcytosine conjugated to Ovalbumin
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS. No preservatives added.
Recognizes human 5-Methylcytosine. Species sequence homology: Mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Habib, M., et al. (1999). Exp Cell Res. 249:46-53. 2.Tweedies, S., et al. (1997). Mol Cell Biol. 17(3):1469-1475. 3. Hernadez-Blazquez, F.J., et al. (2000). Gut. 47:689-693.