Technical Data
ACTN4, NT (Alpha-actinin-4, F-actin Cross-linking Protein, Non-muscle alpha-actinin 4)
Alpha-actinin is a ubiquitously expressed cytoskeletal protein found in numerous actin structures. It consists of an N-terminal actin-binding domain, a central rod domain, and a C-terminal domain; the protein functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins. Alpha-actinin 4 is a non-muscle isoform version of alpha-actinin that is concentrated in the cytoplasm. It plays a role in cell motility, invasion, and metastasis of cancer cells. Alpha-actinin 4 is commonly expressed in filopodia, lamellipodia, stress fibers, and sites of adhesion.

Suitable for use in Immunoprecipitation, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:10,000
Immunocytochemistry: 1:100-1:250
Immunohistochemistry (paraffin): 1:100-1:250
Immunoprecipitation: 1:10-1:100
Optimal dilutions to be determined by the researcher.

Positive Control:
Human fetal skeletal muscle tissue lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20°CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to human ACTN4 at N-terminal.
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Recognizes human ACTN4. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ylänne, et al. (2001). Structure. 9(7):597-604. 2. Entrez Gene: gene-centered information at NCBI. (2005). Nucleic Acids Res. 33:D54-D58. 3. Yamada, et al. (2009). Int J Oral Maxollofac Surg. 4. Otey and Carpen. (2004). Cell Motil. 58:104-111.