Technical Data
029409
AGO1 (Protein Argonaute-1, hAgo1, Argonaute1, Eukaryotic Translation Initiation Factor 2C 1, eIF-2C 1, eIF2C 1, Putative RNA-binding Protein Q99, EIF2C1)
Description:
Ago1 belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. It is required for G1 arrest and mating in response to nitrogen starvation. It is also known as a cell cycle control protein through its regulation of cytokinesis and its related cell cycle checkpoints, which occur downstream of dcr1. It is required, indirectly, for regulated hyperphosphorylation of cdc2. Ago 1 also has a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. It has been shown to be non-essential for siRNA-directed target RNA cleavage, but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k13A152Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to human AGO1 fused to GST-tag.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human AGO1. Does not react with Ago2, Ago3, or Ago4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Azuma-Mukai A., et al. (2008) Proc. Natl. Acad. Sci. USA. 105: 7964-7969. 2. Kim, D.H., et al. (2008). Proc Natl Acad Sci USA. 105(42):16230-16235. 3. Sasaki, T., et al. (2003). Genomics. 82(3):323- 330. 3. Janowski, B.A., et al. (2006). Nat Struct Mol Biol. 13(9):787-792. 4. Ma, J.B., Ye, K., and Patel, D.J. (2004). Nature. 429(6989):318-322.