		Technical Data
029409	AGO1 (Protein Argonaute-1, hAgo1, Argonaute1, Eukaryotic Translation Initiation Factor 2C 1, eIF-2C 1, eIF2C 1, Putative RNA-binding Protein Q99, EIF2C1)

Description:
Ago1 belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. It is required for G1 arrest and mating in response to nitrogen starvation. It is also known as a cell cycle control protein through its regulation of cytokinesis and its related cell cycle checkpoints, which occur downstream of dcr1. It is required, indirectly, for regulated hyperphosphorylation of cdc2. Ago 1 also has a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. It has been shown to be non-essential for siRNA-directed target RNA cleavage, but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a,k	13A152	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Recombinant corresponding to human AGO1 fused to GST-tag.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

Specificity:

Recognizes human AGO1. Does not react with Ago2, Ago3, or Ago4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Azuma-Mukai A., et al. (2008) Proc. Natl. Acad. Sci. USA. 105: 7964-7969. 2. Kim, D.H., et al. (2008). Proc Natl Acad Sci USA. 105(42):16230-16235. 3. Sasaki, T., et al. (2003). Genomics. 82(3):323- 330. 3. Janowski, B.A., et al. (2006). Nat Struct Mol Biol. 13(9):787-792. 4. Ma, J.B., Ye, K., and Patel, D.J. (2004). Nature. 429(6989):318-322.