		Technical Data
029411	AGO4 (Protein Argonaute-4, Argonaute4, hAgo4, Eukaryotic Translation Initiation Factor 2C 4, eIF-2C 4, eIF2C 4, EIF2C4, AGO4, KIAA1567)

Description:
AGO4 also known as EIF2C4 is involved in regulation of gene silencing via small RNA’s such as siRNA and miRNA. This protein contains both a PAZ and a PIWI domain characteristic of Argonaute family of proteins. Functionally Ago4 is the catalytic component of the RNA-induced silencing complex (RISC). As part of this multi-protein complex, Ago4 interacts with miRNAs to help mediate translational repression of targeted messages.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a,k	13A154	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Recombinant corresponding to human AGO4.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

Specificity:

Recognizes human AGO4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Gao, Z., et al. (2010). Nature. 465(7294):106-109. 2. El-Shami, D., et al. (2007). Genes Dev. 21: 2539-2544. 3. Agorio, A. and Vera, P. (2007). Plant Cell. 19(11):3778-3790. 4. Azuma-Mukai, A., et al. (2008). Proc Natl Acad Sci USA. 104(23):7964-7969.