Technical Data
029411
AGO4 (Protein Argonaute-4, Argonaute4, hAgo4, Eukaryotic Translation Initiation Factor 2C 4, eIF-2C 4, eIF2C 4, EIF2C4, AGO4, KIAA1567)
Description:
AGO4 also known as EIF2C4 is involved in regulation of gene silencing via small RNAs such as siRNA and miRNA. This protein contains both a PAZ and a PIWI domain characteristic of Argonaute family of proteins. Functionally Ago4 is the catalytic component of the RNA-induced silencing complex (RISC). As part of this multi-protein complex, Ago4 interacts with miRNAs to help mediate translational repression of targeted messages.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k13A154Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to human AGO4.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human AGO4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gao, Z., et al. (2010). Nature. 465(7294):106-109. 2. El-Shami, D., et al. (2007). Genes Dev. 21: 2539-2544. 3. Agorio, A. and Vera, P. (2007). Plant Cell. 19(11):3778-3790. 4. Azuma-Mukai, A., et al. (2008). Proc Natl Acad Sci USA. 104(23):7964-7969.