		Technical Data
029412	AIF-1 (Allograft Inflammatory Factor 1, AIF-1, Ionized Calcium-binding Adapter Molecule 1, Protein G1, G1, IBA1)

Description:
AIF-1 is an actin-binding protein that enhances the actin-bundling activity of LCP1 in membrane ruffling and plays a role in RAC signaling and in phagocytosis. Studies show that AIF-1 may play a role in macrophage activation and function by promoting the proliferation of T-lymphocytes and lymphocyte migration.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1ug/ml
Immunohistochemistry: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Human lymph node tissue lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic linear peptide corresponding to human AIF-1 (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

Specificity:

Recognizes human AIF-1. Species Crossreactivity: Rat. Species sequence homology: Equine, chimpanzee and rhesus macaque (100%), mouse (89%).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Van Steenwinckel, J., et al. (2011). J Neurosci. 31:5865-5875. 2. Harhausen, D., et al. (2010). J Immunol. 184(11):6407-6410. 3. Stridh, L., et al. (2011). J Neuroinflammation. 8:45.