Technical Data
029412
AIF-1 (Allograft Inflammatory Factor 1, AIF-1, Ionized Calcium-binding Adapter Molecule 1, Protein G1, G1, IBA1)
Description:
AIF-1 is an actin-binding protein that enhances the actin-bundling activity of LCP1 in membrane ruffling and plays a role in RAC signaling and in phagocytosis. Studies show that AIF-1 may play a role in macrophage activation and function by promoting the proliferation of T-lymphocytes and lymphocyte migration.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1ug/ml
Immunohistochemistry: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Human lymph node tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic linear peptide corresponding to human AIF-1 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human AIF-1. Species Crossreactivity: Rat. Species sequence homology: Equine, chimpanzee and rhesus macaque (100%), mouse (89%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Van Steenwinckel, J., et al. (2011). J Neurosci. 31:5865-5875. 2. Harhausen, D., et al. (2010). J Immunol. 184(11):6407-6410. 3. Stridh, L., et al. (2011). J Neuroinflammation. 8:45.