Technical Data
029413
AIF1 (Allograft Inflammatory Factor 1, AIF-1, Ionized Calcium-binding Adapter Molecule 1, Protein G1, G1, IBA1)
Description:
The allograft inflammatory factor-1 (AIF-1) is a 17kD interferon-gamma inducible Ca(2+)-binding EF-hand protein that is encoded within the HLA class III genomic region. AIF-1 is known to be identical with ionized Ca(2+)-binding adaptor 1, microglia response factor-1, and daintain. AIF-1 expression is mostly limited to the macrophage/monocyte lineage, and is augmented by cytokines, such as IFN-gamma. It is reported to be involved in neuronal function, inflammatory responses, allograft rejection, autoimmunity, and the activation and function of macrophages. It is also shown to be expressed in macrophages and microglial cells in autominnume disesases such as experimental autoimmune encephalomyelitis, and neuritis and uveitis models.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Immunohistochemistry: 1:300
Optimal dilutions to be determined by the researcher.

Positive Control:
Human thymus tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k13A155Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic linear peptide corresponding to human AIF1.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human AIF1. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Liu, G., et al. (2007). 40(2):95-102. 2. Del gado, F., et al. (2006). 18(6):588-593. 3. McDaniel, D.O., et al. (2010). 42(10):4235-4237.