Technical Data
029415
Alpha Centractin (ARP1, Alpha-centractin, Centractin, ARP1, Actin-RPV, Centrosome-associated Actin Homolog)
Description:
Alpha centractin is also known as actin related protein 1 (ARP1) is an important element of the dynactin complex which is thought to be involved in the organization of the centrosome and Golgi apparatus and determining nuclear nature with manchette microtubules during spermatid differentiation. It is essential for proper cytoskeleton formation and remodeling during mitosis and alpha-centractin under-expression has been found in the fetal down syndrome cortex.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1ug/ml
Immunohistochemistry: 1:200
Optimal dilutions to be determined by the researcher.

Positive Control:
Human fetal skeletal muscle tissue lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic linear peptide corresponding to human Alpha centractin (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human Alpha Centractin. Species Crossreactivity: mouse and rat. Species sequence homology: chimpanzee (100%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gulesserian, T., et al. (2002). Biochem Biophys Res Commun. 291(1):62-67. 2. Fouquet, J.P., et al. (2000). J Cell Sci. 113: 877-886.