		Technical Data
029418	Aromatase (Cytochrome P450 19A1, CYPXIX, Cytochrome P-450AROM, Estrogen Synthase, CYP19A1, ARO1, CYAR, CYP19)

Description:
Human Aromatase is a membrane-bound enzyme that is localized on the endoplasmic reticulum. While it is found in gonads and adrenal glands where it converts androgens into estrogens in a classical endocrine manner, it is also expressed in various tissues where it regulates local estrogen levels or estrogen/androgen ratios in an intracrine manner. Human Aromatase is known to have two major forms, characterized by one amino acid difference at amino acid position 264 [arginine (264R) and cysteine (264C)]. Abnormal Aromatase hyperactivity is a known cause for Aromatase excess syndrome (AES), which is characterized by excess levels of estrogen, heterosexual precocity in adolescent males and isosexual precocity in adolescent females. On the other hand, Aromatase deficiency (AROD) in Aromatase-deficient (ArKO) mice leads to increased adiposity, an inability to synthesize estrogen, birth defects, and genital-related developmental disorders. It is also reported to play a role in a complex immune-neuro-endocrine network management system in murine cysticercosis.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human placenta tissue lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic linear peptide corresponding to human Aromatase (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

Specificity:

Recognizes human Aromatase. Species sequence homology: Bovine, chimpanzee, rhesus macaque, porcine and canine (100%), rat and mouse (94%).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Kagawa, N. (2011). Methods Mol Biol. 705: 109-122. 2. Tiulpakov, A., et al. (2005). J Clin Endocrinol Metab. 90(7):4184-4190. 3. Jones, M.E., et al. (2000). Proc Natl Acad Sci USA. 97(23):12735-12740. 4. Morales-Mondor, J., et al. (2008). Curr Top Med Chem. 8(5):400-407.