Technical Data
ATG4A (Cysteine Protease ATG4A, AUT-like 2 Cysteine Endopeptidase, Autophagin-2, Autophagy-related Cysteine Endopeptidase 2, Autophagy-related Protein 4 Homolog A, hAPG4A, APG4A, AUTL2)
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. ATG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of target proteins, such as MAP1LC3, GABARAPL2 and GABARAP. ATG4A belongs to the peptidase C54 family, and is widely with high expression level in skeletal muscle and brain.

Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-10,000
Immunoprecipitation: 1:10-1:100
Immunohistochemistry (paraffin): 1:100-1:250
Optimal dilutions to be determined by the researcher.

Positive Control:
Evaluated by Western Blot in fetal liver, HepG2, Jurkat, and fetal brain lysates.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to human ATG4A.
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Recognizes human ATG4A. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Entrez Gene: gene-centered information at NCBI. (2005). Nucleic Acids Res. 33:D54-D58. 2. The UniProt Consortium. (2010). The Universal Protein Resource (UniProt). Nucleic Acids Res. 36:D190-D195.