Technical Data
029426
BMP4 (Bone Morphogenetic Protein 4, BMP-4, Bone Morphogenetic Protein 2B, BMP-2B, BMP2B, DVR4) (FITC)
Description:
Bone morphogenetic protein 4 (BMP4) is a polypeptide belonging to the TGF-B superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signalling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. If these cells also receive signals from FGF, they will differentiate into the spinal cord; in the absence of FGF the cells become brain tissue.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b13A161Affinity Purified
SizeStorageShippingSourceHost
100Tests-20CBlue IceMouse
Concentration:
As reported
Immunogen:
Recombinant corresponding to mouse BMP-4.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% sodium azide, 15mg/ml BSA. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes human BMP-4. Species sequence homology: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Masuhara, K., et al.(1995). Bone;16(1):91-96.